- Concentrates 500 µL down to 15 µL
- Concentration factor 25 x to 30 x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time - 10 to 30 minutes
- Consistent recoveries thanks to the reverse spin capability
- Reverse spin capability provides consistent recoveries
Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange and protein dialysis
- Size exclusion and protein fractionation
The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.