Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
-
Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…
-
Cell Dissociation
CorningTrypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of…
-
Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
-
Leibovitz's L-15
CorningLeibovitz's L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by…
-
Cell Freezing Medium B
Quality BiologicalCell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…
-
Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
-
Iscove's Modified Dulbecco's Medium (IMDM)
Quality BiologicalThis modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate…
-
Lymphocyte Separation Medium (LSM) was originally designed for the in vitro isolation of lymphocytes from diluted whole blood. It is a sterile, iso-osmotic polysucrose and diatrizoate solution with low viscosity and a density of 1.077-1.080 g/mL at 20ºC. Complete Certificate of…
-
Corning Matrigel basement membrane matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in ECM proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycans, entactin, and nidogen. Corning…
-
Corning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…
-
Dulbecco’s Modified Eagle’s Medium - High Glucose
MilliporeSigmawith 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is…
-
Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
-
FlexiPump® Pro Complete Dispensing Assembly
InterscienceThe FlexiPump Pro complete dispensing assembly with a Y connector makes it easy to gain access to various liquid samples without the risk of any harmful consequences.
-
Normal Frog Ringer (NFR)
Ecocyte BioscienceNormal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity. Product information autoclaved Use immediately once opened. Store at 4 - 8 °C Ional composition of NFR Solution…
-
A 96-well insert system with a filter plate pre-coated with structured layers of phospholipids. Design and Materials Microplate type: 96-well microplate configuration complies with ANSI standards Filter plate: Polystyrene with 0.4 µm Polyvinylidene fluoride (PVDF) membrane …
-
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
-
This Tryptic Soy Broth (TSB) product is offered for use in sterility testing and validation in the manufacture of a liquid product. The use of this medium in place of the product intended for production allows an accurate assessment of sterility throughout the manufacture process. It is a sterile,…
-
Corning® glutagro™ DMEM
CorningDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of…
-
Standard Aerobic Culture Medium in Plastic Culture Vials
BECTON DICKINSON AND COMPANY(Continental US Only) (Must be kept Refrigerated; Ships Overnight and on Ice) (Drop Ship Requires Pre-Approval)
-
Corning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…
-
Amino Acids & Vitamins
CorningL-glutamin L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds.…
-
Sodium Pyruvate 100X
Quality BiologicalSodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…
-
Catalase Reagent Droppers are used in a qualitative procedure for determining catalase activity by bacteria. The enzyme catalase is preset in most aerobic and facultatively anaerobic bacteria, with the major exception of streptococci and enterococci. The enzyme catalase decomposes hydrogen…
-
-
The Corning® BioCoat® Poly-D-Lysine coverslip and dish is perfect for enhanced cell attachment and growth of a variety of primary cells as well as transformed cells in serum-free or serum-containing cultures..
-
-
Cellvation Cryopreservation Media
MP BiomedicalsCellvation Cryopreservation Media is animal component free and has been shown to preserve the potential of stem cell for differentiation. This medium is recommended for the preservation of stem cells, hepatocytes, tissue samples, and a wide variety of cell types and established cell lines. …
-
ZymeFree™ Enzyme Free Cell Dissociation Reagent in Dulbecco’s Phosphate Buffered Saline 1X. Chemically defined cell dissociation reagent used for gentle dissociation of adherent cells.
-
HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer. Contains stabililzed dipeptide of L-Glutamine.
-
Dulbecco's Phosphate Buffered Saline 1X w/ Calcium and Magnesium w/o Phenol red. Commonly used for tissue disaggregation and monolayer dispersal.
-
Phosphate Buffered Saline, pH 7.2 1X. Commonly used for tissue disaggregation and monolayer dispersal.