Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
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L-Glutamine, 200mM Solution
Quality BiologicalL-glutamine is a cell culture supplement, and is required in cell culture for cellular growth. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, gluthione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production.…
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RPMI 1640
CorningNamed for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse…
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HiGlutaXL™ Dulbecco’s Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM / F12, 1:1 Mixture) w/ L-Alanyl-L-Glutamine, Sodium bicarbonate and Trace elements w/o HEPES buffer. Contains stabililzed dipeptide of L-Glutamine.
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Artificial Cerebrospinal Fluid (ACSF)
Ecocyte BioscienceArtificial cerebrospinal fluid (aCSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications. Product…
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Leibovitz's L-15
CorningLeibovitz's L15 medium was originally developed without a sodium bicaronate buffer system for use in non-CO2 equilibrated environments. It is buffered by phosphates and the amino acid L-arginine, along with L-histidine and L-cysteine. The formation of acid metabolite byproducts is prevented by…
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Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. in vitro use of collagen can exert effects on the…
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EMEM with L-Glutamine
Quality BiologicalEagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…
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Lymphocyte Serum-Free Medium
CorningCorning's line of specialty media is optimized for those who require media for a wide range of specialized applications and cell lines. The use of T-lymphocytes in adaptive immunotherapy is emerging as an important tool in the treatment of cancer and other clinical applications.…
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EMEM without Phenol Red
Quality BiologicalEMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater…
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Dulbecco’s Modified Eagle’s Medium - High Glucose
MilliporeSigmawith 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is…
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Yeast Extract
CorningYeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates obtained from autolyzed yeast. This is the water soluble portion with intact B-complex vitamins and can be used as an additive for culture media. 10,000 MW cut-off
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Corning BioCoat cell culture inserts are pre-coated with extracellular matrix proteins for applications requiring a protein-coated cell surface, such as cell adhesion, growth, invasion, migration and/or differentiation. Coatings include Corning Matrigel® matrix, Fibronectin, Collagen, and Laminin.
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DMEM:HAMS F-12 1:1 with L-Glutamine
Quality BiologicalDMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…
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1x Park and Terasaki's Modification of McCoy 5A
MP Biomedicals1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and…
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RPMI-1640 with L-Glutamine
Quality BiologicalRPMI was developed to support lymphoblastoid cells in culture, RPMI-1640 has now been successfully used for the culture of a wide range of anchorage independent cells. Although intended for use with serum supplementation, RPMI-1640 has been shown to support the serum-free growth of a number of…
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Corning® Cell Recovery Solution
CorningAllows for recovery of cells cultured on Corning Matrigel Matrix. It depolymerizes Matrigel without enzymatic digestion and lengthy incubation periods at high temperatures for subsequent culturing and/or biochemical analysis of cells. Used to depolymerize Matrigel matrix in 2D or 3D cultures,…
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A 96-well insert system with a filter plate pre-coated with structured layers of phospholipids. Design and Materials Microplate type: 96-well microplate configuration complies with ANSI standards Filter plate: Polystyrene with 0.4 µm Polyvinylidene fluoride (PVDF) membrane …
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Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
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Cefinase™ discs are intended for use in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus species, Haemophilus influenzae, enterococci and anaerobic bacteria for the production of β-lactamase.
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Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen,…
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Tissue Cassettes with Removable Lids
Globe ScientificThe 30° angled cassette features an extra-large writing surface and two side panels ideal for labeling. The cassette has an integral lid that snaps apart and securely locks into the base of the cassette. The lid can be removed and reattached repeatedly to access the specimen. Note: These cassettes…
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Alpha MEM with L-Glutamine
Quality BiologicalAlpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Prepared plated media 100 x 10 mm and 100 x 15 mm plates
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Hanks' balanced salt solution is designed to equilibrate with air, hence does not require CO2 air mixture. All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been…
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Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of…
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Trace Element Solution 2
MP BiomedicalsMP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I. To be added aseptically to media as a supplement. Prior to use of media, it may be necessary to adjust pH and sterile filter (optional, reduces contamination).
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All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt…
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Cell Freezing Medium B
Quality BiologicalCell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…
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Para-Marker
Electron Microscopy SciencesA wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.
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McCoy's 5A Medium with L-Glutamine
Quality BiologicalMcCoy’s 5A Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. With L-glutamine and Phenol Red McCoy’s 5A Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a…