Features
Base material:
- silica 60, specific surface (BET) ~ 500 m2/g, mean pore size 60 Å
- specific pore volume 0.75 mL/g
- particle size 2–10 μm
- for preparative plates (1 mm thickness of layer) standard silica 60
- particle size 5–17 µm
- pH stability 2–10
Indicator:
- acid-resistant product with a pale blue fluorescence for short-wave UV (254 nm)
- UV-absorbing substances apppear as dark-blue to black spots on a light-blue background.
- Partial octadecyl modification, wettable with water, carbon content 7.5 %
Order of polarity: silica > DIOL > NH2 > CN > RP-2 > C18-50 > RP-18 W > C18-100
Normal phase or reversed phase separation modes with eluents from anhydrous solvents to mixtures with high concentrations of water (see figure); the relative polarity of the eluent determines the polarity of the layer.
Recommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates).