PVA coated capillaries contain a permanently adsorbed layer of poly(vinyl alcohol). This coating minimizes hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pH range even under basic conditions from 2.5 to 9.5. This stability allows use of a wide range of common CE buffers. Because the silica surface is covered, many proteins and amines can be analyzed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility can be improved.
Each batch of PVA coated capillaries is rigidly tested by Agilent Technologies and includes a representative electropherogram to assure quality.
The color coding of the capillary (alignment stopper) and the alignment interfaces allows you to easily combine the correct interface with the capillary. Capillaries for non-Agilent CE system users have removable alignment stoppers without color code.
The PVA capillaries for CE/MS have a blue alignment stopper matching the blue color code of the alignment interface for MS-UV Det. The alignment stopper of the 50 µm ID PVA capillary for CE/MS has a black dot for easy identification.
When extended pathlength capillaries are used in non-Agilent systems, loss of resolution may be found if the axial slit width is not reduced. In Agilent systems the alignment interface contains properly matched slits to maintain resolution.
Agilent Technologies products are intended for sale/shipment within the US only. International customers should contact their local Agilent Technologies distributor to place orders for Agilent Technologies products.