For dye samples stable at room temperature
- Complete instructions, background information and study questions included
- Ready-to-load samples
- For 6 gels
Agarose gel electrophoresis is a powerful analytical method for the separation of biomolecules. The Principles and Practice of Agarose Gel Electrophoresis kit, 4313B01, demonstrates the basic procedures, including gel casting and sample application and separation using a selected set of colored dyes that have different properties and various rates of mobility during electrophoresis. Kit includes 1 tube practice gel loading solution, 1 bottle of UltraSpec-Agarose™ Powder, 1 bottle of 50x concentrated electrophoresis buffer, 1 mL pipet, 100 mL graduated cylinder (package for samples) and 10 microtipped transfer pipets.
The Size Determination of DNA Restriction Fragments kit, 4313B15, demonstrates the electrophoretic techniques for the estimation of DNA fragment sizes, which are essential in the mapping of restriction enzyme sites. This experiment introduces the principles of DNA fragment size determination or measurement. It includes standard DNA markers and fragments of unknown size. Kit contains 1 tube practice gel loading solution, 1 bottle of UltraSpec-Agarose Powder, 1 bottle of 50x concentrated electrophoresis buffer, 1 bottle of concentrated Methylene Blue Plus™ Stain, 1 mL pipet, 100 mL graduated cylinder (packaging for samples), 10 micro-tipped transfer pipets and a semi-log graph paper template.
The PCR - Polymerase Chain Reaction kit, 4313B23, introduces students to the principles, practice and applications of Polymerase Chain Reaction (PCR). This simulation experiment does not contain human DNA and does not require a thermal cycler. Kit contains 1 tube practice gel loading solution, 1 bottle of UltraSpec-Agarose Powder, 1 bottle of 50x concentrated electrophoresis buffer, 1 bottle of concentrated Methylene Blue Plus Stain, 1 mL pipet, 100 mL graduated cylinder (packaging for samples) and 10 micro-tipped transfer pipets.
All kits require: Horizontal gel electrophoresis apparatus, DC power supply, staining tray and net, visualization system (white light), automatic micropipets with tips to obtain samples for 6 gels, pipet pump, 250 mL flask or beakers, hot gloves, distilled or deionized water, microwave, hotplate or Bunsen burner, photo-documentation system (optional) and a balance. Kits 4313B01 and B15 also require a waterbath.