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MilliporeSigma

Microcon, Ultracel DNA Fast Flow Membrane

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Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:

  • Typical recoveries of >95%, even for dilute solutions
  • Reverse spin to maximize recovery, even in the smallest samples
  • Convenient storage of filtrate or concentrated sample in standard microfuge tube
  • Concentration factors up to 100X

Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

  • High recovery for small volumes with reverse spin (concentration factor <20X)
  • Low-binding Ultracel® membrane
  • Fast processing

Sample applications:

  • Recovery of genomic DNA for forensic applications
  • Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
  • Removal of primers from amplified DNA
  • Easy filtrate recovery, exploiting the flat membrane design
Description:Microcon, Ultracel DNA Fast Flow Membrane
Key Applications:Protein Extraction, Protein Purification, Sample Prep
Application Notes:Fixed angle rotor: 500 x g for concentration spin; 1,000 x g for reverse spin
Filter Type:Ultrafiltration
Filtration Area, cm²:0.32
Sterility:Non-Sterile
Length, cm (in):4.50 (1.8)
Chemistry:Regenerated Cellulose
Centrifugal Application:DNA Concentration and Desalting
Minimum Final Concentrate Volume, µL:50
Length, mm:45
Hold-up Volume, µL:>10
Diameter, mm:12.3
Volume, mL:0.5
Device Material:

Membrane: Ultracel® low binding regenerated cellulose
Device top: Polycarbonate
Membrane support base: Acetal
Filtrate/concentrate tube: Polypropylene
O-ring: Medical-grade silicone rubber

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Product Detail
Thomas No.
1211L43
Mfr. No.
MRCF0R100
Description
Microcon, Ultracel DNA Fast Flow Membrane
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